Figure 2.

Downregulation of Id2 gene expression by WY is dependent on PPARα. Wild-type SV129 mice (+/+) or SV129 mice that lack PPARα (−/−) were fed a control diet (Control) or a diet containing WY (0.1%) or DEHP (0.6%) for 3 weeks. Total RNA isolated from liver was separated by 1.2% agarose, transferred to nylon, and analysed by northern blot using probes for Id2, Ehhadh, and 17βHSD, and GAPDH as a control. Northern autoradiograms (a) were densitometrically scanned and expression normalized to that of GAPDH (b). Each value represents the mean ± standard error of three animals. *significantly different from control (P < 0.05).