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. Author manuscript; available in PMC: 2012 Jun 12.
Published in final edited form as: J Biol Chem. 2001 Jan 19;276(15):11531–11538. doi: 10.1074/jbc.M011322200

Fig. 3. The effect of 6AU upon galactose induction of GAL1 in wild-type and mutant yeast strains.

Fig. 3

A, Northern analysis was carried out on RNA from DY103 (WILD TYPE), DY106 (dst1), DY105 (rpb2–10), and DY108(rpb2–10 dst1) cells treated with 6AU (75 µg/ml) for 30 min before galactose induction. RNA was prepared at the indicated times, and the blot was probed for GAL1 mRNA. Results from three separate experiments were quantitated using a PhosphorImager. GAL1 transcript levels were corrected for background levels of the same area in each sample lane. These values were divided by the maximal level of GAL1 transcript in wild-type cells at 120 min, and the means and standard deviations (error bars) were plotted (B).