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. Author manuscript; available in PMC: 2012 Jun 12.
Published in final edited form as: J Biol Chem. 2001 Jan 19;276(15):11531–11538. doi: 10.1074/jbc.M011322200

Fig. 4. Glucose shutoff of GAL1 in wild-type and mutant yeast.

Fig. 4

A, Northern blot analysis was performed on RNA harvested at the indicated times after glucose addition to cultures of DY103 (WILD TYPE), DY106 (dst1), DY105 (rpb2–10), and DY108 (rpb2–10 dst1) cells grown for 2 h in galactose. RNA was also prepared from control cells that were not induced with galactose (−). The blot was probed for GAL1 mRNA, and results were quantitated using a PhosphorImager for three separate experiments. GAL1 transcript levels were corrected for background signal in the same area for each lane. Transcript levels for each strain were calculated relative to its zero time value, and the means and standard deviations (error bars) were plotted (B).