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. Author manuscript; available in PMC: 2012 Jun 12.
Published in final edited form as: J Biol Chem. 2001 Jan 19;276(15):11531–11538. doi: 10.1074/jbc.M011322200

Fig. 5. The effect of 6AU upon glucose shutoff of GAL1 in wild-type and double mutant (rpb2–10 dst1) yeast.

Fig. 5

A, Northern blot analysis was performed on RNA from DY103 (WILD TYPE) and DY108 (rpb2–10 dst1) cells grown for 2 h following addition of galactose. 6AU (75 µg/ml) was added for 30 min as indicated, followed by glucose. RNA was prepared at the indicated times as well as before and after induction by galactose (− gal and + gal lanes). The blot was probed for GAL1 mRNA, and results were quantitated using a PhosphorImager for three separate experiments. GAL1 transcript levels were corrected for background signal in the same area for each lane. The GAL1 transcript levels were expressed as the fraction of starting GAL1 transcript remaining for each culture, and the means and standard deviations (error bars) were plotted (B).