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. Author manuscript; available in PMC: 2012 Jun 12.
Published in final edited form as: J Biol Chem. 2001 Jan 19;276(15):11531–11538. doi: 10.1074/jbc.M011322200

Fig. 8. The effect of 6AU upon transcription of the Ia site in wild-type and mutant yeast.

Fig. 8

A, Northern blot analysis was carried out on RNA from DY500, DY510, DY520 (WILD TYPE); DY501, DY511, DY521 (dst1); DY502, DY512, DY522 (rpb2–10); and DY503, DY513, DY523 (rpb2–10 dst1) yeast untreated (−) or treated with 75 µg/ml 6AU (+) for 30 min and challenged with galactose for 1 h. The blot was probed with the PCR product 3′ of the Ia site, shown in Fig. 6. Arrows indicate the full-length transcripts produced by the different plasmids. B, the means and standard deviations (n = 3) were calculated, normalized to the level of transcript in untreated wild-type cells containing pYES2, and plotted.