Table 5.

Summary of antibody dilutions and conditions used in immunoblotting analysis of proteins in transgenic mice and WT mice

Marker	Primary antibody		Secondary antibody
	Species and dilution	Purchased from	Dilution	Purchased from
BACE1	Rabbit polyclonal 1:400	Calbiochem, San Diego, CA, USA	Donkey anti-rabbit HRP 1:10,000	GE Healthcare, Amersham, UK
Amyloid, precursor protein	Rabbit polyclonal 1:500	Abcam, Cambridge, MA, USA	Donkey anti-rabbit HRP 1:10,000	GE Healthcare, Amersham, UK
C-terminal, APP	Rabbit polyclonal 1:400	Millipore, Temecula, CA, USA	Donkey anti-rabbit, HRP 1:6,000	GE Healthcare, Amersham, UK
6E10	Mouse monoclonal 1:300	Covance, San Diego, CA, USA	Sheep anti-mouse, HRP 1:8,000	GE Healthcare, Amersham, UK
A11	Rabbit polyclonal 1:200	Invitrogen Camarillo, CA, USA	Donkey anti-rabbit, HRP 1:6,000	GE Healthcare, Amersham, UK
β-Actin	Mouse monoclonal 1:500	Sigma-Aldrich, St Louis, MO, USA	Sheep anti-mouse HRP 1:10,000	GE Healthcare, Amersham, UK