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. 2012 Apr 5;21(13):3013–3024. doi: 10.1093/hmg/dds131

Figure 5.

Figure 5.

Nrf2 mediates DJ-1-dependent Trx1 expression and cytoprotection. (A) Nrf2 knockdown reduces Trx1 expression. Flag-DJ-1 and empty-vector-engineered cells were transfected with siRNA-targeting Nrf2 (siNrf2), and western blots were performed 72 h later. Quantification of band intensities relative to β-actin from three experiments is shown below. **ANOVA P < 0.01 compared with Flag-DJ-1 and scrambled siRNA-transfected group. (B) Nrf2 knockdown diminishes the protective effect of DJ-1 against H2O2-induced toxicity. Cells were transfected with Nrf2 siRNA for 48 h and then treated with the indicated H2O2 concentrations for 24 h. Cell death was assessed by LDH assay. *ANOVA P< 0.05.