Table 2.
Specific labeled probes used for the detection of AIV genes.
| Sequence (5′ to 3′) | Gene |
|---|---|
| Cy5 atg GAG AAA ATA GTG CTT CTT TTT GCA ATA | HA |
| Alexa 350 atg AAT CCA AAT AAG AAG ATA ATA ACC ATC | NA |
| Fluo atg GCG TCT CAA GGC ACC AAA CGA TCT TAT | NP |
To facilitate binding of probe and labeling with dye, an additional 3 bp (atg) were added to the 5′ end of the primer.