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. 2012 Jun 4;2012:425907. doi: 10.1155/2012/425907

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Copyright © 2012 P. Subramanian et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Recombinant altered PEDF proteins secreted to the culturing media were analyzed by anion-exchange column chromatography. Altered PEDF proteins were K146/K147/R149 (2 K,1R→3A) and K189/K191/R194/K197 (3 K,1R→4A), and FLAG-PEDF. (a) the backbone of the 3D structure of PEDF (tube in grey) from PDB 1IMV with highlighted location for amino acids K146/K147/R149 (blue) and K189/K191/R194/K197 (light blue). FLAG tagged PEDF had two tandem repeats of DYKDDDDK on the carboxy-end (C(A)). (b) Conductivity of PEDF fractions at peaks by anion-exchange column chromatography was determined and plotted.