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Effect of PEDF proteins on wound healing assay: MDA-MB-231 (a) and ARPE-19 (b) cell monolayers were scratched with a 10-μL pipette tip to make a wound. Cells were then incubated with media alone or PEDF proteins (20 ng/mL) for 48 h in (a) and 18 h in (b). Images were taken at the end points and compared to 0 h to measure wound healing.
