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. 2012 Jun 11;197(6):789–800. doi: 10.1083/jcb.201110049

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© 2012 Liem et al.

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Figure 4. — Ultrastructure of wild-type and mutant neural tube cilia. (A–F) Longitudinal sections (left) and cross-sections of cilia (top right) and basal bodies (bb; bottom right) from the neural tube in wild type (WT) and mutants. (A) Wild-type cilia have a 9 + 0 doublet microtubule organization in the axoneme and triplet microtubules in the basal body. (B and C) The microtubules in Ift144^twt (B) and Ift144^dmhd/twt (C) mutant cilia appear normal, whereas the cilia are slightly wider than wild type. (D) Ift144^dmhd mutant cilia lack axonemal microtubules and contain vesicle-like structures and have a normal-appearing basal body. (E) Longitudinal sections show that neural tube Dync2h1^mmi cilia are swollen and filled with arrays of electron-dense particles that resemble trains of IFT particles. A few singlet microtubules can be seen in cross-sections of Dync2h1^mmi cilia (arrow). (F) The cilia of Ift144^twt Dync2h1^mmi double mutants are less swollen than the Dync2h1^mmi single mutants and do not accumulate IFT trains but show a more severe phenotype than Ift144^twt cilia. Cilia are from E9.5 (wild type and Ift144^dmhd) and E10.5 (Ift144^twt, Ift144^dmhd/twt, Dync2h1^mmi, and Ift144^twt Dync2h1^mmi) embryos. Bars, 200 nm.