Figure 2. Conserved region driven β-galactosidase activity in the orofacial region and limb.

(A) Schematic of the transgenic reporter construct used in transient transgenic analyses. The black rectangle denotes the location of CONS region insertions upstream of the human beta globin promoter in pGLKS (see Materials and Methods). (B) Schematic of the Bmp4^lacZneo allele [45] (white boxes, exons; gray boxes, coding regions). (C) Number of transient transgenic embryos with various CONS derivative constructs that supported β-galactosidase expression in limb or orofacial tissue.