Figure 3. Signaling downstream of FGFR1 in 94-10 cells.

A. The Human Phospho-Kinase Array was performed to examine FGFR1 signalling. Boxes 1, 2 and 3 represent activated proteins from the MAPK, STAT and downstream effectors of MAPK induced signaling cascades, respectively. B. Confirmation by Western blotting of the phosphorylated proteins identified in the Human Phospho-Kinase Array. Vector controls and FGFR1-expressing cells were cultured with heparin and FGF2 for indicated time periods (minutes). Lysates were harvested and used for Western blotting. C. Western blots showing inhibition of specific pathways using small molecule inhibitors. 94-10-FR1 cells were cultured with BAPTA-AM, U0126 or BIRB for 1 hr prior to addition of FGF2 for 10 min.