Table 2. The reductive activity of wild-type and variant POR expressed alone and the activity of POR co-expressed with CYP3A4 or CYP2B6 in sf9 was estimated by NADPH-dependent Cytochrome c reduction.
| Protein | POR (µmol/min/mg protein) | CYP3A4-POR (µmol/min/mg protein) | CYP2B6-POR (µmol/min/mg protein) | |||
| Vector | 0.04±0.002* | (1%) | – | – | ||
| Wild-type | 3.9±0.32# | (100%) | 4.5±0.21 | (100%) | 4.7±0.15 | (100%) |
| K49N | 3.3±0.21# | (85%) | 3.5±0.29 | (78%) | 4.5±0.25 | (97%) |
| A115V | 2.7±0.15# * | (69%) | 3.1±0.13 | (69%)* | 4.3±0.37 | (93%) |
| Y181D | 0.5±0.07# * | (13%) | 2.3±0.15 | (50%)* | 2.6±0.11 | (56%)* |
| S244C | 4.4±0.35# | (113%) | 5.8±0.31 | (130%) | 5.3±0.18 | (113%) |
| A287P | 0.8±0.06# * | (21%) | 2.5±0.13 | (54%)* | 2.9±0.43 | (63%)* |
| G413S | 6.9±0.54# * | (177%) | 8.7±0.41 | (193%)* | 9.9±0.43 | (211%)* |
The activity of each POR mutant was calculated and expressed as a percentage of the activity of wild-type POR, arbitrarily set at 100%.
The values mean ±S.D. of three independent experiments.
#
: p<0.01 in comparison with the vector control cells.
*
: p<0.01 in comparison with the cells expressing wild-type (WT) POR.