Table 1. Characteristics of the amplicons submitted to HRM analysis.

Segment	Primer Sequences 5′–3′	Samples	Variant	GenBank Accession Number	Amplicon Size
5	GIM seg5 5F ATGGATGGTCTAAATACAACTTC	GF-025	Insert IN4	EU130923	98
	GIM seg5 1R ACAGCATTTGATGAACTCTTCTC	GF-797 GF-603	No insert Q266	EU851044	65
		GF-552 GF-918	No insert L266	GU830907	65
6	GIM seg6 3F TCATGAGGGAGGTAGCATTG	GF-797 GF-603*	HPR0	EU118820	192
	GIM seg6 5R CAATCCCAAAACCTGCTACAC	GF-552	HPR2	AF364889	132
		GF-041	HPR5	EU625673	129
		GF-025	HPR7b	FJ594319	123
		GF-918	HPR8	AY973192	120

^*The sequence is identical to EU118820 except for the single nucleotide change of A to C (Figure 1B *).