Table 2. Experimental Melting Temperature for amplicons in segment 5 and 6.
Segment | Melting peak Temperature °C | |||||
5 | Insert IN4 | No Insert Q266 | No insert L266 | |||
77.94±0.07 | 76.45±0.14 | 76.35±0.00 | ||||
6 | HPR0_1 | HPR0_2 | HPR2 | HPR5 | HPR7b | HPR8 |
81.69±0.0 | 81.02±0.11 | 80.28±0.10 | 79.95±0.0 | 78.20±0.10 | 80.43±0.10 |