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. 2012 Jun 12;7(6):e37265. doi: 10.1371/journal.pone.0037265

Table 2. Experimental Melting Temperature for amplicons in segment 5 and 6.

Segment Melting peak Temperature °C
5 Insert IN4 No Insert Q266 No insert L266
77.94±0.07 76.45±0.14 76.35±0.00
6 HPR0_1 HPR0_2 HPR2 HPR5 HPR7b HPR8
81.69±0.0 81.02±0.11 80.28±0.10 79.95±0.0 78.20±0.10 80.43±0.10