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. 2012 Jun 12;7(6):e38429. doi: 10.1371/journal.pone.0038429

Figure 2. Validation of genes related to the TNF (TNF) and TNF receptor (TNFR) superfamilies (SF).

Figure 2

A. Compared with the HD group, the expression levels of the genes related to the TNFSF and TNFRSF in the LTBL, LTBH and TB groups were analyzed using a clustering algorithm. Eight genes (TRAIL, BAFF, CD30, APRIL, LTBR, TNFR1, TRAILR3 and CD40) were clustered as an identical category characterized by TB specific up-regulation. B. Using real-time PCR, we validated the eight TB-specific genes and detected seven genes that were significantly increased in the peripheral CD4+ T cells of TB patients. The mRNA values of the validated genes were normalized to the housekeeping gene β-actin. The numbers of participants included in validation test were the following: HD, n = 10; LTBL, n = 12; LTBH, n = 7; TB, n = 10. The validation results among the seven genes of the pentagram are marked BAFF and APRIL in Figure 3.A.