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. 2012 Jun 12;7(6):e39038. doi: 10.1371/journal.pone.0039038

Figure 6. Analysis of cytokine secretion by PBMCs following SIV-restimulation.

Figure 6

At the indicated time points, PBMC were incubated with AT2-inactivated SIV, microvesicles, or SEB as control. Culture supernatants were harvested after 48 h and concentrations of IFN-γ were determined by ELISA. Data obtained with microvesicles were subtracted, and means and SEM are shown (#, p<0.05 compared with C and D, †, p<0.05 compared with A; one-way ANOVA followed by Bonferroni Post test; *, p<0.05 for differences to baseline; two-way ANOVA). Substantial concentrations of IL-4, IL-10, or IL-17 were not detected in supernatants of SIV-stimulated cells derived from either group (data not shown).