Figure 1. GW788388 administration at 3 dpi decreased parasitemia and heart inflammatory infiltrates and increased survival rates.

Male Swiss mice were injected IP with 10⁴ bloodstream trypomastigotes. Then GW788388 (3 mg/kg) or DMSO was administered by gavage at 3 dpi. (A) Parasitemia was measured by direct counting of parasites in blood. (B) Percent survival was monitored during the experiment until 30 dpi. (C–F) At 15 dpi, mice were sacrificed and heart sections stained with hematoxylin-eosin were analyzed by light microscopy. Numerous amastigote nests (C, open arrows) and large inflammatory infiltrates (E, filled arrows) were observed in untreated T. cruzi-infected mice. GW788388 administration decreased the number of amastigote nests (D, open arrow) and of inflammatory infiltrates (F). (G and H) The mean number of amastigote nests in 30 fields (G) and the area (%) of inflammatory infiltrates (more than 10 mononuclear cells) (H) are shown. Values for the infected group treated with GW788388 that were significantly different from the value for the DMSO infected group are indicated (**p<0.01 and * p<0.05). n = 10 mice/group in 4 independent experiments.