Figure 4. GW788388 administration at 3 dpi inhibited connexin 43 disruption.

Male Swiss mice were injected IP with or without (NI) 10⁴ bloodstream trypomastigotes. Then GW788388 (3 mg/kg) (Y GW788388) or DMSO (Y DMSO) was administered by gavage at 3 dpi. (A–C) At 15 dpi, mice were sacrificed and heart sections stained with anti-Cx43 antibody (green, Cx43 plaques are indicated by white arrows) and DAPI (nuclei, colored in blue). Quantitative analysis of the number of Cx43 plaques (D) and length (E), on images from each group studied (NI, Y DMSO and Y GW788388). Values are expressed as the mean±SD (F) 20 µg of total proteins from hearts at 15 dpi were resolved in 12% SDS/PAGE and immunoblotted with anti-Cx43 and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibodies. (G) Densitometric histograms of the normalized levels of Cx43 as related to GAPDH are shown. Values that were significantly different from studied groups are indicated by asterisks (* p<0.05). n = 3 mice/group in 3 independent experiments.