Skip to main content
. Author manuscript; available in PMC: 2012 Jun 12.
Published in final edited form as: Nature. 2011 Sep 14;477(7364):335–339. doi: 10.1038/nature10400

Figure 2. Increased caspase-8 independent cell death within crypts of Casp8ΔIEC mice.

Figure 2

(a) Representative pictures of gut organoids. Inset = Eosin staining indicating Paneth cells. Graph: Number of Paneth cells (pc) per organoid crypt (n=24) +SEM. (b) Crypt cross sections from the small intestine of control and Casp8ΔIEC mice stained with H&E. TUNEL ( Inset: condensed nuclei) and caspase-3. (c) Quantification of necrotic cells per crypt + SD of control (n=9) and Casp8ΔIEC (n=14) mice. (d) Electron microscopic pictures of dying crypt cells in Casp8ΔIEC mice and inducible Casp8ΔIEC. Asterisks, Paneth cell granules; “n”, nuclei; “l”, crypt lumen; Arrows: mitochondrial swelling.