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Role of Src in mediating the microenvironmental effect on the FRET response of the RhoA biosensor. A,B) Time courses of normalized Venus/ECFP emission ratio (mean ± SEM) of the RhoA biosensor in response to EGF in A) RMCs and B) SMCs pretreated with (“PP1”) (the cell numbers for RMC and SMC are 4 and 6, respectively) or without PP1 (“Control”) (the cell numbers for RMC and SMC are 9 and 6, respectively), as indicated.
