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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: DNA Repair (Amst). 2012 May 24;11(7):624–635. doi: 10.1016/j.dnarep.2012.05.001

Fig 6. Recruitment of flag tagged RECQL5 fragments to the laser-induced DSB sites.

Fig 6

(A) Graphic image of Flag tagged RECQL5 fragments. The fragments 1-533 and 534-991 include an artificial NLS at the C-terminus. The full length and flag-tagged RECQL5 fragments; 1-533, 534-991 and 853-991 were transfected in HeLa cells (B) and U2OS cells (C) and fixed 5 min after micro-irradiation at 9% laser intensity. The arrows in white indicate the site of the laser hit and recruitment of these flag-tagged RECQL5 fragments. The fragment 853-991 is excluded from the recruitment. The fragments containing the helicase and KIX domains are recruited.