(A) A channel recording demonstrating the effect of IGF-1 (200 nM) on the Cl channels in the presence of genistein (10 μM). The channel closed level is indicated by a dotted line and C. The holding potential was −60 mV (hyperpolarization). (B) The bar graph summarizes the results of experiments in which the effect of IGF-1 on Cl channels was examined in the absence or in the presence of Herbimycin A or genistein (control NPo, 0.98±0.1; IGF-1, 0.24±0.05; genistein, 1.01±0.1; IGF+genistein, 1.03±0.1; Herbimycin A, 0.97±0.08; IGF+Herbimycin A, 0.99±0.1) (n=5–6). “#” indicates a significant difference in comparison to the control.