(A) A channel recording demonstrating the effect of IGF-1 (200 nM) on the Cl channels in the presence of LY294002 (10 μM). (B) The bar graph summarizes the results of experiments in which the effect of IGF-1 on Cl channels was examined in the absence or in the presence of LY294002 or Wortmannin (control NPo, 1.02±0.1; IGF-1, 0.23±0.05, Ly294002(LY), 1.08±0.1; IGF+LY, 1.06±0.1; Wortmannin, 1.15±0.08; IGF+Wortmannin, 1.08±0.1) (n=5–7). “#” indicates a significant difference in comparison to the control. (C) A channel recording demonstrating the effect of IGF-1 (200 nM) on the Cl channels in the presence of wortmannin (100 nM). The experiments were performed in cell-attached patch and the holding potential was −60 mV.