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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Gene Ther. 2012 Mar 8;20(2):158–168. doi: 10.1038/gt.2012.16

Figure 1.

Figure 1

Rat MAPC expansion and viability during 3D aggregate culture. (a) Cell proliferation in the aggregate during 5 days in growth media. Data are shown as mean ±SD of eight independent experiments. (b) Cell viability under fluorescence microscopy in one 3D aggregate using a non-fluorescent compound that changes to a red fluorescent color when cells are viable and metabolically active and changes to green when cells are damaged or dead. The image shown is a merge of three images taken with bright field, TRITC and FITC filters. (c) Three-dimensional rendered image showing viable cells (red) in a 3D aggregate under fluorescence microscopy. (d) Aggregate growth in diameter during 5 days in growth media. Data are shown as mean ±SD of five independent experiments. *p<0.01 when statistically compared to baseline (day 0 or 1).