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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Xenobiotica. 2012 Mar 8;42(8):748–755. doi: 10.3109/00498254.2012.662726

Figure 2. Cellular distribution of BCRP by confocal laser microscopy.

Figure 2

LN229 cells and MCF-7 cells were transfected with control and BCRP siRNAs for 24 h. Cells were then fixed and immunolabeled with monoclonal anti-BCRP (green, upper panels) and polyclonal anti-emerin (red, middle panels) antibodies. Merge images are shown in the bottom panels. Areas of nuclear co-localization between BCRP and emerin appear in yellow in LN229 cells, which were absent in MCF-7 cells. Similar results were obtained in 3 independent experiments. (B) Vertical sections in the XY and XZ planes of confocal images from LN229 and MCF-7 cells tranfected with control siRNA. Magnification 40X.