Figure 4. HDL mimetic, L-4F reduces viability, inhibits proliferation and affects cell cycle and cyclin proteins in CT26 cells.
CT26 cells were cultured as described in Materials and Methods, and incubated with either vehicle (control) or L-4F at a concentration of 10μg/mL. (A) Cells were assayed for viability using the MTS assay kit. P < 0.001. (B) BrdU incorporation was analyzed as described under Materials and Methods. P < 0.001. (C) Quantitative analysis of cells in different phases in cell cycle. Data are represented as the mean ± SD of the percent of control cells. (D) The expression of Cyclin D1 and Cyclin A. All experiments were performed in triplicate and each assay was carried out in quadruplicates.