Figure 6.

pRb is the effector of Pin1 in G1/S cell cycle control. (a) WB analysis of PIN1/RB1 KD T98G cells. (b) pRb is the effector of Pin1. Cell proliferation was assessed as in Figure 2a; ^**P<0.01. (c) Colony-forming unit assay. Cells were grown for 2 weeks and stained with methylene blue. (2) Scrambled, (3) PIN1 KD, (4) pRb KD, and (5) PIN1/pRb double KD cells. (d) Average of five experiments as in (c). ^*P<0.05, ^**P<0.01. (e) Scrambled (black) and pRb KD (white) cells were treated with indicated dose of Juglone (^*P<0.05). (f) T98G scrambled and PIN1 KD cells were immunoprecipitated with E2F1 antibody. In PIN1 KD cells, there is more interaction between pRb and E2F1