Figure 7.

Pin1 controls pRb phosphorylation in different cancer cells. (a) MCF7 and OVCAR3 PIN1 KD cells showed hypophosphorylated pRb as in T98G cells. WB was stained with anti-Pin1, anti-p-pRb-S780, and anti-pRb. (b) Kinase assay performed as in Figure 1e. CDK/cyclin complexes were immunoprecipitated from MCF7 and OVCAR3 lysate cells (^*P<0.05, ^**P<0.01). (c) GST-Pin1 interacts with pRb in MCF7 and OVCAR3 cells. (d) FACS analysis of OVCAR3 cells as in Figure 2c. (e) pRb is hypophosphorylated in PIN1 KD OVCAR3 cells. Overexpression of Pin1, but not the catalytically inactive form, restores pRb phosphorylation in PIN1 KD cells. Cells were analyzed by WB with pRb and pRb-S780 phospho-specific antibodies. P, phosphorylation. The membrane was normalized with α-tubulin