Figure 2.

CRD domain-containing C-terminus of GODZ is necessary for the interaction with DR4. (a) Schematic diagram of GODZ functional domains. Numbers indicate amino-acid residues of GODZ. (b and c) Mapping of GODZ domains for the interaction with DR4. HEK 293T cells were transfected with pDR4-Flag (DR4-Flag) alone or together with either pGODZ-HA or pGODZ deletion mutant for 24 h, and cell extracts were IP with anti-HA antibody (b) or anti-Flag (M2) antibody (c). The immunoprecipitates were then analyzed by western blotting using anti-Flag or anti-HA antibody. WCL are indicated and heavy chains (^*) of immunoglobulins are served as loading control. TM, transmembrane domain; CRD, cysteine-rich domain