Figure 3.

Reduced growth of Ksr1 deficient Eμ-myc pre-B cells. (a) Bone marrow from littermates of the indicated genotype was placed into culture on day 0. Viable cells were counted by Trypan Blue Dye exclusion at intervals, and population doublings were calculated. A representative experiment is shown; similar results were obtained with bone marrow from littermates from different parents. (b and c) Equal numbers of Ksr1+/+ Eμ-myc, Ksr1+/− Eμ-myc, and Ksr1−/− Eμ-myc pre-B cells were placed in culture and viability was assessed by Trypan Blue Dye exclusion (b) and MTT assay (c). (d) Myc and β-actin protein levels in littermate-matched pre-B cells were determined by western blot