Figure 8.

Effect of TG2 transamidating activity on the clearance of ubiquitinated protein aggregates. (a) Western blot analysis of p62 and ubiquitinated proteins in HEK293, HEK293^TG2 and HEK293^C277S cell lines subjected to immunoprecipitation for TG2. Cells treated with MG132 for 2 h were lysed and proteins were immunoprecipitated using anti-TG2 antibody. Immuno- and co-immunoprecipitated proteins were separated by SDS-PAGE and immunoblotted using the indicated antibodies. Arrows indicate ubiquitinated proteins present in TG2 interacting protein complexes. (b) Immunoblotting analysis of ubiquitinated proteins in HEK293, HEK293^TG2 and HEK293^C277S cell lines. Cells were incubated with MG132 for 2 h, and ubiquitin expression levels were assayed by immunoblotting with anti-ubiquitin antibody. Actin was used as loading control