Table 1.
The best gradient elution program: A: 0.1 % formic acid in water, B: acetonitrile
| Time (min) | Solvent | Flow rate (mL/min) | |
|---|---|---|---|
| A (%) | B (%) | ||
| 0.0 | 100 | 0 | 2.0 |
| 1.0 | 98 | 2 | 2.0 |
| 2.0 | 80 | 20 | 2.0 |
| 3.0 | 40 | 60 | 2.0 |
| 3.5 | 20 | 80 | 2.0 |
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