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. 2012 Jun 13;2:456. doi: 10.1038/srep00456

Figure 3.

Figure 3

Scheme of (a) the two-step assay for visible detection of bacteria using biotinylated antibody (b-Ab) as SLs to control the extent of aggregation of stAuNPs. (b) Relative difference between the size of the cell and that of NPs on switching b-Abs off when they attach on the cell surface. (c) Shift of REVC in response to the presence of E. coli, when tests performed using biotinylated antibody (b-Ab) as SL in 400 μL total sample volume with a fixed stAuNPs concentration (absorption: 0.43 @ 531 nm for 1/10 diluted sample) and. E.coli cell loads are typical of several tests, which yielded similar results.