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Representative thin-layer chromatography (TLC) immunostaining. TLC immunostaining was performed on all the phospholipids simultaneously, as reported previously [7]. Lane A, reactivity of the serum from a patient positive for both cardiolipin (CL) and phosphatidylethanolamine (PE); lane B, reactivity of the serum from a patient positive for lyso(bis)phosphatidic acid antibodies (LBPA); lane C, no background staining was observed with the serum from a healthy subject.
