Fig. 7.

Interferon (IFN)-γ-dendritic cell (DC) primed T cells are antigen-specific in their suppressive function. IFN-γ-DC-primed T cells were isolated from primary mixed leucocyte reaction (MLR) and co-cultured with a fixed number of autologous naive CD4⁺ T cells (10⁵) at varying ratios of 1 naive to 1 primed (1:1), 2:1, 4:1 or 8:1 and stimulated with UT-DC at a DC to naive T cell ratio of 1:10. At 1:1 naive to primed T cell co-cultures, an irrelevant third-party DC was used to determine the antigen specificity of regulatory T cells. Cells were cultured for 5 days and proliferation was determined by [³H]-thymidine incorporation (counts per minute). Mean ± standard deviation *P < 0·0004. Figure representative of four independent experiments.