Fig. 8.

Early interferon (IFN)-γ exposure of FAST-dendritic cells (DC) do not have a significantly different gene expression profile compared to late IFN-γ exposure. DC were either treated with IFN-γ at time 0 h (early) or exposed to IFN-γ after 24 h of differentiation (late), or were left untreated (no). All DC were generated in the presence of interleukin (IL)-4 and granulocyte–macrophage colony-stimulating factor (GM-CSF). DC were first stained for CD83, to confirm differences in IFN-γ exposure. (a) Representative of three independent experiments. Total RNA from DC samples were extracted and analysed by a human Affymetrix gene array chip for differences in gene signature, hierarchical clustering was used to generate a heat map for data visualization (b). Analysis based on four independent experiments. Genes of greater than 20-fold increase in comparison to UT-DC were graphed, based on step-up statistical analysis (c).