Figure 2 .

Immunoblot analysis of amounts of PaMpk1 and PaMpk2 (MAPK) and phosphorylation (phospho-MAPK) in wild type throughout the life cycle. Extracts from ΔPaMpk1 and ΔPaMpk2 mycelia were loaded as controls. Mycelium of 3-day-old WT cultures was separated into three zones corresponding to 1 day of growth (1), 2 days (2), and 3 days (3) or extracted as a whole (mycelium). Gel loading with extract corresponding to 3 days contained more proteins. PaMpk1 and PaMpk2 are present and phosphorylated in all three zones in roughly equal amounts. They are also present and phosphorylated in maturing 2-day- (perithecia 2 d) and mature 4-day-(perithecia 4 d)-old fruiting bodies. They are present in an unphosphorylated form in ascospores not induced for germination (NI) and PaMpk2 is phosphorylated upon induction of germination (I).