. 2012 Jun;191(2):493–507. doi: 10.1534/genetics.112.138768

Table 1 . The delayed development of apl-1(yn5) mutants requires daf-12 and daf-16 activity.

Strain (genotype)	N_eggs	L1 (%)	L2 (%)	L3 (%)	L4 (%)	Adult (%)	N_worms (T)	Average survival 20° (%) ± STE	N_eggs (T)	L1 (%)	L2 (%)	L3 (%)	L4 (%)	Adult (%)	Dauer (%)	Average survival 25° (%) ± STE
Wild type (N2)	3462	0	0	0	3	97	3572 (30)	103 ± 0.7	1310 (9)	0	0	0	75	25	0	104 ± 3.0
ynIs106 [APL-1EXT(yn32)]	256	0	0	0	0	100	259 (3)	98 ± 10.7	349 (3)	0	0	0	94	6	0	100 ± 1.1
apl-1(yn5)	943	1	2	10	82	5	826 (11)^a	86 ± 1.7^a	694 (6)^a	4	13	51	32	0	0	75 ± 2.1^a
ynIs71 [APL-1EXT]	488	1	1	2	37	59	397 (6)^a	82 ± 4.5^a	283 (3)^a	9	29	60	2	0	0	64 ± 8.6^a
daf-12(m20)	936	0	0	0	1	99	921 (7)	97 ± 7.3	581 (5)^a	20	66	10	4	0	0	90 ± 4.5
daf-12(m20); ynIs71	244	0	0	0	7	93	197 (3)	80 ± 6.2^a^,^b	253 (5)^a	24	65	6	5	0	0	75 ± 3.7^a^,^b
N2 grown on L4440 RNAi	320	0	0	0	0	100	323 (3)	99 ± 1.5
N2 grown on daf-12 RNAi	766	0	0	0	0	100	733 (3)	95 ± 3.1
daf-12(m20) grown on L4440 RNAi	366	0	0	0	0	100	332 (3)	91 ± 5.4
daf-12(m20) grown on daf-12 RNAi	460	0	0	0	0	100	404 (3)	89 ± 5.9
apl-1(yn5) grown on L4440 RNAi	683	1	0	4	95	0	538 (3)^a	78 ± 4.8^c
apl-1(yn5) grown on daf-12 RNAi	743	0	0	0	0	100	608 (3)	81 ± 6.5^c
daf-16(mu86)	579	0	0	0	1	99	597 (5)	100 ± 1.6	953 (11)	1	2	5	92	0	0	98 ± 1.9
daf-16(mu86); apl-1(yn5)	1272	8	5	3	11	73	1115 (4)^d	85 ± 7.0^a^,^e	838 (8)^d	5	2	14	79	0	0	36 ± 4.3^a^,^d
zIs356 [DAF-16::GFP]	2241	0	1	9	90	0	2236 (12)^a	99 ± 1.8	468 (5)^a	7	0	0	0	0	93	81 ± 3.8^a
zIs356 [DAF-16::GFP]; apl-1(yn5)	1165	1	26	60	13	0	1031 (3)^a^,^d^,^f	90 ± 4.9^a	781 (7)^a^,^d^,^f	73	24	0	0	0	3	79 ± 4.6^a
daf-2(e1370)^g	748	1	0	2	93	1	691 (4)^a	92 ± 7.7	545 (5)^a	3	0	0	0	0	97	89 ± 5.2
daf-2(e1370); apl-1(yn5)^h	416	10	13	77	0	0	283 (3)^a^,ⁱ	69 ± 11.2^a^,^d^,ⁱ	504 (5)^a^,^d^,ⁱ	92	0	0	0	0	8	42 ± 5.2^a^,^d^,ⁱ
daf-2(e1370); daf-16(mu86)^h	299	0	1	1	3	95	271 (3)ⁱ	91 ± 5.7	354 (3)ⁱ	2	1	4	93	0	0	83 ± 2.5^a^,^e
daf-2(e1370); daf-16(mu86); apl-1(yn5)^h	371	6	8	2	19	65	254 (3)^d^,ⁱ	68 ± 8.9^a^,^d^,^e^,ⁱ	209 (3)^d^,ⁱ	17	2	8	73	0	0	47 ± 14.6^a^,^d^,^e^,ⁱ

Developmental stage of animals was scored 72 and 48 hr after eggs were laid at 20° and 25°, respectively. All developmental distributions are shown in cumulative form. Is alleles indicate integrated transgenes. Transgenes shown in brackets. Transgenes were driven by the apl-1 promoter unless otherwise noted; the encoded protein is indicated; DAF-16::GFP is driven by the daf-16 promoter. ynIs106 carries a transgene that contains the apl-1(yn32) missense mutation in the region encoding the extracellular domain; this transgene does not rescue apl-1(yn10) null lethality and is considered a nonfunctional APL-1EXT control. N = number of animals observed. T = number of independent times experiment was performed. L4440 RNAi, empty vector control. Note that Gems et al. (1998) reported that daf-2(e1370); daf-12(m20) animals at 25.5° were found as L1 (43%) and L2 or L3 (57%), but no dauers or adults were observed. SUR-5::GFP was used as a co-injection marker for ynIs71 and ynIs86, Pmyo-2::GFP for ynIs106, and rol-6(su1006) for ynIs79 and ynIs100. For statistical analysis: P-values were determined by one-way ANOVAs with Tukey post-test (95% confidence intervals) for survival rate and χ² (4 d.f.) for developmental progression and are only indicated when P < 0.001. For P-values against controls at the indicated temperature, see footnotes ^a–f and ⁱ. STE, standard error.

wild type

daf-12(m20)

wild type on RNAi L4440

apl-1(yn5)

daf-16(mu86)

zIs356 [DAF-16::GFP]

form on average 3% dauers

no dauers observed at 20°

ⁱ

daf-2(e1370)