Table 2 . APL-1 overexpression causes a temperature-sensitive lethality.
| 20° | 25° | 27° | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Strain (genotype) | Neggs | Nworms | T | Average % survival STE | % L1 | Neggs | Nworms | T | Average % survival STE | % L1 | Neggs | Nworms | T | Average % survival STE | % L1 |
| Wild type (N2) | 3462 | 3572 | 30 | 103 ± 0.7 | 0 | 1310 | 1351 | 9 | 104 ± 3.0 | 0 | 5633 | 6006 | 50 | 106 ± 1.6 | 1 |
| ynIs107 [APL-1(yn32)D342C/S362C::GFP] | 518 | 528 | 4 | 102 ± 1.4 | 0 | 810 | 816 | 8 | 100 ± 1.9 | 1 | |||||
| ynIs100 [apl-1(yn32)::GFP] | 386 | 394 | 4 | 102 ± 2.3 | 0 | 575 | 621 | 6 | 106 ± 2.3 | 1 | |||||
| ynIs106 [apl-1(yn5 yn32)] | 256 | 259 | 3 | 98 ± 10.7 | 0 | 349 | 352 | 3 | 101 ± 1.1 | 0 | 316 | 311 | 3 | 98 ± 1.4 | 0 |
| ynIs86 [APL-1] | 455 | 404 | 5 | 88 ± 4.5 * | 0 | 420 | 315 | 4 | 77 ± 4.7 * | 0 | 449 | 265 | 4 | 60 ± 3.5 * | 31 |
| ynIs79 [APL-1::GFP] | 1015 | 639 | 10 | 63 ± 5.6 * | 1 | 494 | 266 | 5 | 34 ± 10.8 * | 3 | 1690 | 64 | 14 | 4.3 ± 0.9 * | 47 |
| ynIs71 [APL-1EXT] | 488 | 397 | 6 | 82 ± 4.5 * | 1 | 283 | 180 | 3 | 64 ± 8.6 * | 9 | 484 | 261 | 5 | 54 ± 2.9 * | 72 |
| apl-1(yn5) | 943 | 826 | 11 | 86 ± 1.7 * | 2 | 694 | 512 | 6 | 75 ± 2.1 * | 4 | 3737 | 1841 | 41 | 47 ± 1.9 * | 46 |
| apl-1(yn5); yn38 | 668 | 635 | 5 | 95 ± 4.8 | 2 | 497 | 480 | 5 | 96 ± 1.4 ** | 23 | |||||
| apl-1(yn5); yn39 | 364 | 237 | 3 | 69 ± 10.9 *,** | 8 | 586 | 117 | 5 | 18 ± 2.9 *,** | 69 | |||||
Is alleles indicate integrated transgenes. Transgenes shown in brackets. Transgenes were driven by the apl-1 promoter unless otherwise noted; the encoded protein is indicated; DAF-16::GFP is driven by the daf-16 promoter. apl-1(yn32) is a missense mutation that corresponds to a null allele. N = number of animals observed. T = number of independent times or trials experiment was performed. SUR-5::GFP was used as a co-injection marker for ynIs71 and ynIs86, Pmyo-2::GFP for ynIs106 and ynIs107, and rol-6(su1006) for ynIs79 and ynIs100. For statistical analysis: P-values were determined by one-way ANOVAs with Tukey post-test (95% confidence intervals) for survival rate and are only indicated if P < 0.001 against wild type (*) or apl-1(yn5) (**) at the given temperature. STE, standard error.