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. 2012 Jun;191(2):509–521. doi: 10.1534/genetics.111.137844

Figure 2.

Figure 2

Mutations in egl-8 strongly suppress the synaptic defects of ttx-7 mutants. (A) Schematic of the head region of C. elegans and a pair of RIA. (B) Schematic of pre- and postsynapses distribution in RIA (White et al. 1986). The localization index was calculated using the equation shown here. Measurement of fluorescent intensity was performed as described in Materials and Methods. (C–E) Comparison of the localization indices of SNB-1::VENUS (C), GFP::SYD-2 (D), and GLR-1::GFP (E) in the RIA neuron in each genotype. Mutations in egl-8 strongly suppressed the localization defects of the synaptic proteins in ttx-7 mutants. Note that egl-8(n488) suppressed more strongly than egl-8(nj77) (C). Steel–Dwass multiple comparison tests were performed (n ≥ 11 animals). The marks on the bars of each genotype indicate comparisons with wild type. The marks on the lines indicate comparisons between indicated genotypes. (F) Representative images of the distribution of each synaptic protein in the RIA neuron in each genotype. Solid arrowheads indicate ectopic fluorescence, and open arrowheads indicate absence of the ectopic fluorescence. Scale bar, 5 µm. (G and H) Schematic of SNB-1::VENUS localization in wild-type animals, ttx-7(nj50);egl-8(n488) and egl-8(n488) mutants (G) and that in ttx-7(nj50) mutants (H). SNB-1::VENUS is mislocalized at the proximal region of the process in ttx-7(nj50) mutants. (I and J) Schematic of GFP::SYD-2 localization in wild-type animals, ttx-7(nj50);egl-8(n488) and egl-8(n488) mutants (I) and that in ttx-7(nj50) mutants (J). GFP::SYD-2 mainly localized to the distal region of RIA in wild-type animals, ttx-7(nj50);egl-8(n488) and egl-8(n488) mutants, while it dispersed in whole process in ttx-7(nj50) mutants. (K and L) Schematic of GLR-1::GFP localization in wild-type animals and ttx-7(nj50);egl-8(n488) and egl-8(n488) mutants (K) and that in ttx-7(nj50) mutants (L). GLR-1::GFP mainly localized in the proximal region of RIA in wild-type animals, ttx-7(nj50);egl-8(n488) and egl-8(n488) mutants, while it diffused in whole process in ttx-7(nj50) mutants.