Table 2.
Proteins identified with the highest Mascot scores following LC-MS/MS analysis from each excised DiGE gel spot. The “dye ratio” represents Cy5/Cy3 and −Cy3/C5 intensity ratios for proteins of higher and lower abundance, respectively, in chronic pancreatitis (Cy5) with respect to chronic abdominal pain controls (Cy3).
| Spot# | Mascot result | Calculated | Estimate from gel | dye ratio |
|||||
|---|---|---|---|---|---|---|---|---|---|
| protein name | IPI number | score | peptides | MW (kDa) | pI | MW (kDa) | pI | ||
| 1 | Desmoplakin | IPI00013933 | 426 | 57 | 332 | 6.4 | 250 | 5.7 | 1.4277 |
| 2 | Serum albumin | IPI00745872 | 541 | 28 | 69 | 5.9 | 250 | 5.7 | 2.7033 |
| 3 | Serum albumin | IPI00745872 | 176 | 16 | 69 | 5.9 | 250 | 5.7 | 2.3701 |
| 4 | Endoplasmin | IPI00027230 | 262 | 28 | 92 | 4.8 | 98 | 4.8 | −2.7721 |
| 5 | Serum albumin | IPI00745872 | 441 | 25 | 69 | 5.9 | 98 | 5.7 | 3.0376 |
| 6 | Serum albumin | IPI00745872 | 328 | 23 | 69 | 5.9 | 98 | 5.7 | 2.9618 |
| 7 | Serum albumin | IPI00745872 | 443 | 28 | 69 | 5.9 | 98 | 5.7 | 3.5638 |
| 8 | Serum albumin | IPI00745872 | 446 | 30 | 69 | 5.9 | 98 | 5.7 | 2.6246 |
| 9 | Serum albumin | IPI00745872 | 441 | 26 | 69 | 5.9 | 98 | 5.7 | 3.6763 |
| 10 | TF Serotransferrin | IPI00022463 | 174 | 15 | 77 | 6.8 | 85 | 6.2 | 4.7389 |
| 11 | TF Serotransferrin | IPI00022463 | 71 | 4 | 77 | 6.8 | 85 | 6.2 | 6.2057 |
| 12 | Serum albumin | IPI00745872 | 309 | 19 | 69 | 5.9 | 56 | 5.7 | 2.7793 |
| 13 | Serum albumin | IPI00745872 | 477 | 34 | 69 | 5.9 | 56 | 5.7 | 3.4765 |
| 14 | Serum albumin | IPI00745872 | 567 | 34 | 69 | 5.9 | 56 | 5.7 | 2.858 |
| 15 | Serum albumin | IPI00745872 | 550 | 40 | 69 | 5.9 | 56 | 5.7 | 3.2367 |
| 16 | SNC73 protein | IPI00386879 | 63 | 5 | 53 | 6.5 | 56 | 5.7 | 2.7797 |
| 17 | SNC73 protein | IPI00386879 | 82 | 5 | 53 | 6.5 | 56 | 5.7 | 2.1518 |
| 18 | TF Serotransferrin | IPI00022463 | 620 | 42 | 77 | 6.8 | 56 | 5.7 | 2.476 |
| 19 | Serum albumin | IPI00745872 | 433 | 34 | 69 | 5.9 | 56 | 5.7 | 3.7413 |
| 20 | Serum albumin | IPI00745872 | 613 | 39 | 69 | 5.9 | 56 | 5.7 | 2.7902 |
| 21 | Actin | IPI00021439 | 108 | 6 | 42 | 5.3 | 56 | 5.7 | 1.8866 |
| 22 | Serum albumin | IPI00745872 | 466 | 31 | 69 | 5.9 | 56 | 5.7 | 4.0114 |
| 23 | Serum albumin | IPI00745872 | 346 | 30 | 69 | 5.9 | 56 | 5.7 | 2.8934 |
| 24 | Serum albumin | IPI00745872 | 346 | 30 | 69 | 5.9 | 56 | 4.8 | −4.208 |
| 25 | Protein disulfide-isomerase | IPI00010796 | 184 | 16 | 57 | 4.8 | 56 | 4.5 | −4.401 |
| 26 | Alpha-1-antichymotrypsin | IPI00550991 | 72 | 3 | 51 | 5.3 | 56 | 4.8 | −3.9172 |
| 27 | Pancreatic alpha-amylase | IPI00025476 | 342 | 22 | 58 | 6.6 | 50 | 6.2 | −1.4011 |
| 28 | Pancreatic alpha-amylase | IPI00025476 | 436 | 20 | 58 | 6.6 | 50 | 6.4 | 1.0452 |
| 29 | Alpha-amylase 2B | IPI00021447 | 111 | 4 | 58 | 6.6 | 37 | 4.8 | 1.5795 |
| 30 | Alpha-1-antitrypsin | IPI00553177 | 229 | 10 | 47 | 5.4 | 37 | 4.8 | 1.7298 |
| 31 | Alpha-1-antitrypsin | IPI00553177 | 115 | 10 | 47 | 5.4 | 37 | 4.8 | 1.4306 |
| 32 | Serum albumin | IPI00745872 | 99 | 12 | 69 | 5.9 | 34 | 5.5 | 1.7838 |
| 33 | Serum albumin | IPI00745872 | 255 | 19 | 69 | 5.9 | 34 | 5.5 | 1.792 |
| 34 | Glyceraldehyde-3-phosphate dehydrogenase | IPI00219018 | 81 | 12 | 36 | 8.6 | 29 | 8.0 | −2.1575 |
| 35 | Pancreatic alpha-amylase | IPI00025476 | 132 | 9 | 58 | 6.6 | 25 | 6.4 | −2.4613 |
| 36 | Alpha-amylase 2B | IPI00021447 | 146 | 6 | 58 | 6.6 | 25 | 6.4 | −3.0721 |
| 37 | Serum albumin | IPI00745872 | 251 | 20 | 69 | 5.9 | 18 | 5.0 | −2.5469 |
| 38 | Pancreatic alpha-amylase | IPI00025476 | 158 | 7 | 58 | 6.6 | 20 | 6.4 | 1.163 |
| 39 | Pancreatic alpha-amylase | IPI00025476 | 127 | 5 | 58 | 6.6 | 17 | 5.8 | −1.1942 |
| 40 | Trypsin-1 | IPI00011694 | 287 | 28 | 27 | 6.1 | 16 | 7.2 | −1.5565 |
| 41 | Serum albumin | IPI00745872 | 130 | 7 | 69 | 5.9 | 16 | 7.7 | −1.1998 |
| 42 | Trypsin-1 | IPI00011694 | 283 | 29 | 27 | 6.1 | 16 | 6.5 | −1.5206 |
| 43 | Serum albumin | IPI00745872 | 180 | 12 | 69 | 5.9 | 16 | 6.2 | −1.2733 |
| 44 | Pancreatic alpha-amylase | IPI00025476 | 132 | 6 | 58 | 6.6 | 16 | 6.4 | −1.3937 |
| 45 | Trypsin-1 | IPI00011694 | 67 | 6 | 27 | 6.1 | 16 | 6.6 | −1.1982 |
| 46 | Pancreatic alpha-amylase | IPI00025476 | 173 | 7 | 58 | 6.6 | 12 | 6.2 | 1.4043 |
| 47 | Serum albumin | IPI00745872 | 175 | 15 | 69 | 5.9 | 12 | 5.8 | 2.5705 |
| 48 | Alpha-amylase 2B | IPI00021447 | 108 | 4 | 58 | 6.6 | 12 | 5.8 | 1.9996 |
| 49 | Alpha-amylase 2B | IPI00021447 | 148 | 5 | 58 | 6.6 | 12 | 5.8 | 1.9578 |
| 50 | Serum albumin | IPI00745872 | 181 | 16 | 69 | 5.9 | 10 | 4.8 | −1.7044 |
| 51 | Serum albumin | IPI00745872 | 211 | 20 | 69 | 5.9 | 12 | 5.8 | 1.876 |
| 52 | Serum albumin | IPI00745872 | 191 | 16 | 69 | 5.9 | 10 | 4.8 | −1.7595 |
| 53 | Pancreatic triacylglycerol lipase | IPI00027720 | 58 | 4 | 51 | 6.3 | 10 | 7.0 | −2.1418 |
| 54 | Carboxypeptidase B | IPI00009826 | 106 | 4 | 47 | 6.2 | 10 | 7.8 | −1.8589 |
| 55 | Pancreatic alpha-amylase | IPI00025476 | 82 | 3 | 58 | 6.6 | 15 | 6.0 | −2.7219 |
| 56 | Alpha-amylase 1 precursor | IPI00025476 | 70 | 4 | 58 | 6.6 | 15 | 6.0 | −2.2757 |
| 57 | Serum albumin | IPI00745872 | 155 | 12 | 69 | 5.9 | 15 | 6.0 | −3.0823 |
| 58 | Pancreatic alpha-amylase | IPI00025476 | 141 | 7 | 58 | 6.6 | 15 | 6.0 | −1.992 |
| 59 | Alpha-2-macroglobulin | IPI00478003 | 71 | 6 | 163 | 6.0 | 7 | 4.4 | −4.1582 |
| 60 | Serum albumin | IPI00745872 | 137 | 13 | 69 | 5.9 | 7 | 4.4 | −3.8268 |
| 61 | Trypsin-1 | IPI00011694 | 84 | 6 | 27 | 6.1 | 8 | 7.6 | 1.1487 |
| 62 | Trypsin-1 | IPI00011694 | 88 | 6 | 27 | 6.1 | 8 | 6.6 | −1.45 |
| 63 | Serum albumin | IPI00745872 | 85 | 10 | 69 | 5.9 | 7 | 8.7 | −2.6158 |
| 64 | Arp2/3 complex 20 kDa subunit | IPI00554811 | 92 | 6 | 20 | 5.3 | 7 | 8.7 | −3.2895 |
| 65 | Serum albumin | IPI00745872 | 211 | 15 | 69 | 5.9 | 7 | 8.7 | −3.2561 |
Abbreviations: Spot#, gel spot as numbered in Figure 3; score, Mascot score; peptides, number of peptides identified per protein; MW, molecular weight; pI, isoelectric point.