Figure 1.

Generation and characterization of the podocin-GFP transgenic zebrafish line showing that the GFP expression overlaps with the endogenous podocin expression. (A) The Tol2 transposon-based construct used to generate the line. A 2.5-kb zebrafish podocin 5′ sequence was cloned into the pT2KXIG plasmid. The flanking Tol2 transposon sites are indicated (red boxes). (B) GFP expression driven by the podocin promoter recapitulates endogenous podocin expression pattern. GFP is exclusively expressed in the pronephric glomerulus (arrowheads; note that fluorescence signals in yolk and eyes represent autofluorescence), illustrated in dorsal and lateral views. The location of GFP is further confirmed by in situ hybridization using GFP and podocin probes (note that the GFP in situ hybridization signal in the head represents unspecific cross-reaction). (C) Cross-sections show the presence of GFP mRNA (left panel) at the location typical for podocytes, as confirmed by the stained obtained using a podocin probe (right panel). nc, notochord.