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. 2012 Jun;10(3):250–259. doi: 10.1089/adt.2011.0406

Fig. 6.

Fig. 6.

OI-RD image of a ligand microarray (68×160 spots) acquired after washing the microarray with 1 × PBS buffer, but before further reaction of the microarray. The image pixel size is 20 μm and the center-to-center spacing of the spots is 250 μm vertically and 225 μm horizontally. The red lines in the top panel show the outlines of 16 identical subarrays and the inset shows the detailed spot layout of each. Each target type was printed in quadruplicate in six different printing concentrations ranging from 16 μM (left) to 0.5 μM (right). BSA borders and unprinted spots are also included as controls. B-BSA, biotin-BSA; Dig, digoxin; DNP, 2,4-dinitrophenol; Glc, glucose; GT a-HM, polyclonal goat IgG against human IgG; GT a-MS, polyclonal goat IgG against MS; GT a-RB, polyclonal goat IgG against rabbit IgG; HM, human IgG; IB, iminobiotin; Mal, maltose; Metal, metallothionein; Meth, Methamphetamine; Morph, morphine; PB, phenobarbital; RB, rabbit IgG; THC, tetrahydrocannabinol; THP, theophylline.