Fig. 6.

Effect of rabeprazole and U73122 on DNA repair in live cells induced by ionizing radiation (IR). HeLa cells were pretreated with 50 μM rabeprazole or U73122 for 20 min at 37°C followed by 100 Gy IR. Cells were then harvested at indicated time points. The chromosome DNAs were analyzed by pulsed field gel electrophoresis. The DNAs were stained with SYBR Green and analyzed by FUJI Fluorescent Imager FLA5000. Damaged DNA was quantitated by the ImageJ software. The average of two independent experiments is shown.