Table 7. Sequence analysis of 10 discrepant rifampin-susceptibility results*.
Sample no. | Rifampin susceptibility |
||
---|---|---|---|
LiPA result | Phenotypic result | Conclusion after sequencing | |
1–3 | Sensitive | Resistant | From the same patient; wild-type rpoB test region |
4 | Sensitive | Resistant | Mycobacterium bovis; wild-type rpoB test region |
5–7 | Resistant (DS4) | Sensitive | From the same patient; synonymous substitution (R528R) not associated with rifampin resistance |
8 | Resistant (DS1) | Sensitive | 2 genotypes present: wild-type (predominant) and mutant (L511P) |
9 | Resistant (R5) | Sensitive | S531L mutation; wild-type rpoB on retesting, thus likely laboratory error |
10 | Resistant (DS2) | Sensitive | D516A mutation; no high-level resistance when seen alone |
*LiPA, line probe assay.