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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Bone. 2011 Sep 20;50(1):42–53. doi: 10.1016/j.bone.2011.09.038

Fig. 6.

Fig. 6

Impaired osteocyte maturation and lacunar-canalicular system in CSF-1KO mice. Toluidine blue stained sections (A), TEM (B,C) and SEM (D,E) images of WT and CSF-1KO bone. A) WT shows well-organized trabecular and cortical bone with osteocytes in lacunae, whereas CSF-1KO shows poor demarcation between trabecular and cortical bone and several empty lacunae (arrows). B,C) WT osteoblasts are layered along bone surfaces with one side adjacent to the bone surface indicating their polarity, whereas CSF-1KO osteoblasts are irregularly shaped and disorganized. Micropetrosis was identified in CSF-1KO by mineralization of lacunar spaces (arrow). WT osteocytes were embedded in well-mineralized matrix and showed well-developed dendritic processes (arrows). Osteocytes in CSF-1KO were surrounded by patchy mineralized matrix (arrowhead) and showed poorly formed dendrites (arrows). D) Backscattered SEM and E) SEM of acid-etched, resin-casted osteocyte lacunar-canalicular system. Note that CSF-1KO osteocyte lacunae are disorganized, decreased in number and have rough walls. There is also a large amount of osteoid present in the matrix. TB, trabecular bone; CB, cortical bone, OB, osteoblast; OC, osteocyte.