Table 2.

Pre-Steady-State Kinetic Parameters for Incorporation of dAMP Opposite dT by wt and Y567A RB69pol.^a,^c

RB69 pol	P/T b	P/T Details	kpol (s−1)	Kd,app (µM)	kpol / Kd (µM−1 s−1)	Ratio
wt	SeqA1	Control	296	36	8.2
wt	SeqA2	3DA at n-1 (T)	61	665	9.1 × 10−2	1/90
wt	SeqA3	3DA at n-1 (P)	38	670	5.7 × 10−2	1/144
wt	SeqA4	3DA at n-2 (P)	10	1100	9.1 × 10−3	1/901
wt	SeqA5	3DA at n-3 (P)	293	38	7.7	1/1.1
Y567A	SeqA1	Control	290	40	7.2
Y567A	SeqA2	3DA at n-1 (T)	283	42	6.7	1.0

^aThe standard deviation for the K_d,app values was ±15%. The standard deviation for k_pol values was ±10%.

^bSequence of P/T was shown in Fig 2A.

^cRepresentative gels, progress curves and plots of k_obs vs [dATP] with SeqA1 and SeqA2 were shown in Fig S1.