. Author manuscript; available in PMC: 2013 May 29.

Published in final edited form as: Biochemistry. 2012 May 17;51(21):4343–4353. doi: 10.1021/bi300416z

Table 3.

Fluorescence Lifetimes of tC^o in Duplex DNA and pol-P/T Binary Complex. ^a,^c

RB69 pol	P/T ^b	P/T Details	Fluorescence lifetimes
RB69 pol	P/T ^b	P/T Details	τ₁ (ns)	τ₂ (ns)	τ₃ (ps)	chi²
	SeqB1	Control			3.8 (1.0)	1.04
	SeqB2	3DA at n-1 (T)			3.9 (1.0)	0.98
wt	SeqB1	Control	4.3 (0.29)	6.4 (0.34)	39 (0.37)	1.05
wt	SeqB2	3DA at n-1 (T)	4.3 (0.19)	7.6 (0.25)	23 (0.56)	1.03
wt	SeqB3	dA/dA at n-1	4.3 (0.19)	7.0 (0.16)	19 (0.65)	1.07

The standard deviation for the lifetime values was ±15%. The number is the parenthesis is the corresponding amplitude for the lifetime species.

Sequences of P/Ts are shown in Fig 2B.

Representative fluorescence decay of wt RB69pol with SeqB2 is shown in Fig S2.